Abstract
The effect of replacement of glucose in glucose-methanol extender (GM) with either sucrose and trehalose on the sperm motility characteristics of cryopreserved semen and the post-thaw storage of salmon, brown trout, brook trout, sex-reversed female rainbow trout and whitefish was investigated. Semen samples were diluted 1:5 or 1:9 (for sex-reversed females) in 9% methanol containing 0.18M glucose, sucrose or trehalose. After thawing (40°C, 5s), sperm was stored for 0, 30, 60, 120 and 240min at 4°C and assessed for sperm motility. The type of sugar had no effect on the percentage of sperm motility of brown trout (65–73%), brook trout (51–58%) or sex-reversed female rainbow trout (38–40%). Glucose and trehalose secured the highest post-thaw sperm motility after thawing (0min) in salmon (64±7%) and whitefish (52±8%), respectively. The longest period (determined as the last time point when the percentage of motility did not significantly change) was recorded for cryopreserved semen of brook trout (60–120min). The shortest was recorded for whitefish semen (30min). Our results suggest that glucose, sucrose or trehalose can be used interchangeably for the cryopreservation of brown trout, brook trout and sex-reversed semen. However, the best results in terms of post-thaw sperm motility were achieved for salmon with glucose and sucrose and for whitefish with trehalose and sucrose. Cryopreserved sperm can be stored for prolonged periods of at least 30min without a loss in sperm motility, which can facilitate more organised hatchery work. Statement of relevanceThe results of this study confirmed the high level of utility of cryopreservation employing a 0.18M glucose and 9% methanol extender for freezing, among Salmonidae species. We have demonstrated for the first time the utility of this method for freezing Atlantic salmon semen. Furthermore it was shown that disaccharides are more efficient than glucose for preserving of whitefish post-thaw motility.
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