Abstract
The possibility of using sugar beet pulp to produce yeast biomass was evaluated in this study. Viscozyme and Ultraflo Max preparations were used for hydrolysis, which was performed on a semi-industrial scale. Out of 250kg of wet beet pulp, 375dm3 of hydrolysate was obtained. After being enriched with mineral salts (N, P, K, Mg), the medium was inoculated with Trichosporon cutaneum LOCK 0254, Candida tropicalis LOCK 0007 Pichia stipitis LOCK 0047, Candida guilliermondii ATCC 6260 and Saccharomyces cerevisiae LOCK 0132 yeast strains.Shaken cultivations were carried out for 48h at 30±1°C. The highest biomass yield was observed for the C. tropicalis strain – 12.1(±0.42)gDMdm−3 medium. The protein content in this biomass was 52.3% of DM. Up to 54.7gDM of the yeast biomass was obtained after 10h of cultivation in the bioreactor. Sugar beet pulp can thus successfully be used for the production of yeast biomass containing considerable amounts of single cell protein.
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