Uticaj skladištenja uzoraka pljuvačke na koncentraciju biohemijskih markera

Abstract

Salivary analysis is rapid, simple and non invasive, so it is often used as a sample for measuring levels of different biomarkers. Storage of saliva samples can influence their concentration. Aim: To investigate the influence of different storage conditions of saliva samples on the level of various biomarkers. Material and methods: Samples of unstimulated saliva were collected from 34 healthy volunteers, male and female, 25­70 years old, with the special test tubes - Salivette. We analyzed glucose, urea, uric acid, triglycerides, calcium and iron, using spectrophotometric method. Sodium and potassium in saliva were measured by flame photometry. Biomarkers' analysis was done in native samples of saliva, then after seven days of storage on +4 oC, finaly after thirty days of storage on ­20 oC. Results: Mean values of biomarkers' concentrations in native saliva were similar as results of other authors. After 7 days of storage on +4 oC, levels of glucose (0,66 mmol/L), urea (5,3 mmol/L), uric acid (228 µmol/L), triglycerides (0,27 mmol/L), calcium (2,11 mmol/L), iron (8,5 µmol/L), potassium (14,9 mmol/L) and sodium (10,3 mmol/L) didn't show statistically significant difference (p > 0,05) related to their concentrations in native saliva samples. Also, mean values of investigated biomarkers weren't statistically different in samples of native saliva and those stored 30 days on ­20 oC. Conclusion: Various storage conditions didn't influence on investigated biomarkers' levels. Stability of these biomarkers shows that saliva has big potential as a diagnostic fluid.