Abstract
Lannea acida (Anacardiaceae), commonly called Kikié in the Noun division (West-Cameroon), is a tree whose bark is used locally to facilitate delivery. This study was aimed at evaluating the in vitro uterotonic effects of aqueous and methanol extracts of L. acida in Wistar rats. Uterine strips isolated from rats pretreated with 5μg estradiol (48h) were mounted in a single-organ bath containing aerated and thermostated De Jalon solution (37°C). After equilibration, non-cumulative effects of L. acida extracts were recorded after application. The effect of the methanol extract (the most active extract) was monitored in the presence of atosiban (a competitive antagonist of oxytocin receptors), atropine (a specific type 3 muscarinic receptor antagonist), nifedipine (an L-type calcium channel antagonist), and 2-Aminoethoxydiphenyl borate (2-ADB, a specific antagonist of inositol 1,4,5-triphosphate receptors type 1), and in calcium-free medium containing EGTA to elucidate its mechanism of action. L. acida induced uterine contraction in a concentration-dependent manner with the methanol extract (1.506 ± 0.032 gf) being the most effective. Administration of atosiban (2μmol/L) and atropine (1μmol/L) reduced the contractile effect of L. acida. Complete inhibition was observed with nifedipine, 2-APB, and calcium-free medium containing EGTA. These results suggest that L. acida possesses uterotonic effects mediated through oxytocin receptors with mobilization of extracellular calcium.
Highlights
Lannea acida (Anacardiaceae), commonly called Kikié in the Noun division (WestCameroon), is a tree whose bark is used locally to solve difficult childbirth
A dose-dependent contractile effect on the rat myometrial strips was recorded after application of aqueous extract of L. acida (Fig. 1A)
The maximum contractile effect was observed at moderate concentration (2.18 mg) while the highest concentration (4.36 mg) was less active
Summary
Lannea acida (Anacardiaceae), commonly called Kikié in the Noun division (WestCameroon), is a tree whose bark is used locally to solve difficult childbirth. This study aimed to evaluate the in vitro uterotonic effects of aqueous and methanolic extracts of L. acida in female Wistar rats. Parturition is the process ofdelivery of a fully-grown fetus on the completion of the normal pregnancy period. This biological process is characterized by an increase in myometrial contractility and the dilatation of the uterine cervix [1]. Caesarian sections can be required [7]. These modern technics are limited due to postpartum bleeding commonly associated to uterotonic compoundsand financial and esthetic consequences of the surgical solution[8]. In developing countries where the accessibility of health services is limited, people largely rely on medicinal plant for primary health problems [9]
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