Uso potencial de extractos de Creosote bush (Larrea tridentata) como desinfectante sobre Salmonella typhimurium en instalaciones avícolas

Abstract

espanolSe evaluaron extractos de hojas de Creosote bush para control de Salmonella typhimurium en instalaciones avicolas. Se determinaron el disolvente y la tecnica que obtuvieran la mayor proporcion de material extractable, in vitro; se determino su capacidad bactericida y se evaluo la capacidad para desinfectar instalaciones avicolas, comparando desinfeccion con Creosote (DC), desinfeccion de rutina (DR) y vacio sanitario (VS). Al termino de la desinfeccion, se colectaron muestras en piso, pared, techo y cortina. Se sembraron en medio selectivo, se realizaron pruebas bioquimicas diferenciales y se calcularon las unidades formadoras de colonia (UFC). El analisis de varianza se realizo con el procedimiento GLM y la comparacion de medias por Tukey, con el programa SAS. Usando agua y proceso de decoccion, con 40 minutos de extraccion, se obtuvo el mayor porcentaje de material extractable (36,43%), la menor concentracion bactericida (300 mg mL–1) y halos de inhibicion de 13 mm. En la pared disminuyeron las UFC con DC, en techo y cortinas fueron similares con DC y DR, en piso fueron menores con DR. Se concluye que el extracto acuoso de hojas de Creosote bush es una alternativa de desinfeccion eficaz para control de Salmonella typhimurium en instalaciones avicolas EnglishExtracts of Creosote bush leaves were evaluated for control of Salmonella typhimurium in poultry pens. Solvent and the technique that obtained the highest proportion of extractable material, in vitro, were determined; its bactericidal capacity was determined and the capacity to disinfect poultry pens was evaluated, comparing disinfection with Creosote bush (DC), routine disinfection (DR) and sanitary vacuum (VS). At the end of the disinfection, samples were collected in floor, wall, ceiling and curtain. Seeded in selective medium, differential biochemical tests were performed and colony forming units (CFU) were calculated. The analysis of variance was performed with the GLM procedure and the comparison of means by Tukey, with the SAS software. Using water and the decoction process, with 40 minutes of extraction, the highest percentage of extractable material (36.43%), the lowest bactericidal concentration (300 mg mL–1), and inhibition halos of 13 mm were obtained. On the wall, the CFU with DC decreased, while in ceiling and curtains they were similar with DC and DR and in floor, lower with DR. It is concluded that the aqueous extract of Creosote bush leaves is an effective disinfection alternative for control of Salmonella typhimurium in poultry pens