Abstract
One of the causes of male infertility is Genital tract infections (GTI). Considering the importance of GTI, widespread recognition of them seems necessary. we aimed to characterize and compare semen microbial populations in fertile and infertile men who referred to an infertility clinic in Yazd, Iran. Semen samples were collected from two groups of fertile (268) and infertile (210) men. Sperm analysis (concentration, morphology, viability and motility parameters) were performed according to the World Health Organization (WHO) 2010 guidelines laboratory manual. Bacterial isolation was performed in Sheep Blood Agar and Eosin Methylene Blue (EMB) agar plates. For PCR, samples were analyzed with genus specific primers. All semen characteristics were poor in the infertile group compared to those in the fertile men (p-value< 0.05). Enterococcus spp. (18.7%, 17.1%; p= 0.814), E. coli (7.9%, 11.4%; p= 0.486), Staphylococcus aureus (6.4%, 2.9%; p= 0.398) and Proteus mirabilis (0%, 2.9%; p= 0.002) were the most common agents, respectively. Also, the results obtained from PCR were confirmed using culture-base method. Proteus mirabilis contamination was identified in the infertile group. While no significant association was observed between male infertility and semen microbial populations, p. mirabilis may be the leading cause of reproduction impairment in men.
Highlights
Male infertility is a worldwide health problem and its etiology is not well defined [1]
Scientific reports suggests that the mechanism of contamination process on male reproductive tract and fertility potential are related to sperm agglutination, sperm immobilization, sperm DNA fragmentation[by increasing in reactive oxygen species (ROS) level],induction of chronic inflammations which can lead to reductions in sperm parameters such as count, motility and morphology [9,10,11]
There was no significant difference in distribution of different microorganisms between the fertile and infertile groups
Summary
Male infertility is a worldwide health problem and its etiology is not well defined [1]. Genital tract infection (GTI) has been suggested as one of the harmful causes for male fertility [7, 8]. Scientific reports suggests that the mechanism of contamination process on male reproductive tract and fertility potential are related to sperm agglutination, sperm immobilization, sperm DNA fragmentation[by increasing in reactive oxygen species (ROS) level],induction of chronic inflammations which can lead to reductions in sperm parameters such as count, motility and morphology [9,10,11]. One of the causes of male infertility is Genital tract infections (GTI). Methods: Semen samples were collected from two groups of fertile [268] and infertile [210] men. Results: All semen characteristics were poor in the infertile group compared to those in the fertile men (p-value< 0.05). While no significant association was observed between male infertility and semen microbial populations, p. mirabilis may be the leading cause of reproduction impairment in men
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