Abstract
Optokinetic response (OKR) is a behavior that an animal vibrates its eyes to follow a rotating grating around it. It has been widely used to assess the visual functions of larval zebrafish1-5. Nevertheless, the standard protocol for larval fish is not yet readily applicable in adult zabrafish. Here, we introduce how to measure the OKR of adult zebrafish with our simple custom-built apparatus using a new protocol which is established in our lab. Both our apparatus and step-by-step procedure of OKR in adult zebrafish are illustrated in this video. In addition, the measurements of the larval OKR, as well as the optomotor response (OMR) test of adult zebrafish, are also demonstrated in this video. This OKR assay of adult zebrafish in our experiment may last for up to 4 hours. Such OKR test applied in adult fish will benefit to visual function investigation more efficiently when the adult fish vision system is manipulated.Su-Qi Zou and Wu Yin contributed equally to this paper.
Highlights
Optokinetic response (OKR) is a behavior that an animal vibrates its eyes to follow a rotating grating around it
We introduce how to measure the OKR of adult zebrafish with our simple custom-built apparatus using a new protocol which is established in our lab
The measurements of the larval OKR, as well as the optomotor response (OMR) test of adult zebrafish, are demonstrated in this video. This OKR assay of adult zebrafish in our experiment may last for up to 4 hours
Summary
If your lab has constructed an OKR apparatus, the key of adult zebrafish OKR is to hold the fish. The drum around fish chamber is 8.5 cm in out diameter, 7.5 cm in inner diameter and 7.0 cm in high. 1. A sponge as shown in Fig.[1] composed by two parts. 5. Put the zebrafish on the sponge with dorsal up and allow one side of its pectoral fin before the first pin. The fifth pin is sited between the first and the third pin and the sixth pin is on the other side of it They could prevent zebrafish from shaking its body. All the pins are just used to prevent zebrafish from shaking its body, too tight will be harmful to fish. The seventh and eighth pin which are planted in the site near gill should not be shelter from their sight of grating
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