Using the ITS2 transcribed spacer of nuclear ribosomal DNA to identify species of the genus Nitraria

Evgeny V Banaev,Tatiana A Poliakova,Taigana Ak-Lama,Mariya A Tomoshevich,E.V Banaev,M.A Tomoshevich,Y.G Zaytseva

doi:10.1051/bioconf/20202400009

Evgeny V Banaev, Tatiana A Poliakova + Show 5 more

Open Access

https://doi.org/10.1051/bioconf/20202400009

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Abstract

Intragenomic polymorphism of ITS2 of nuclear ribosomal DNA sequences was analysed in 30 samples belonging to Nitraria schoberi, N. sibirica. The nucleotide variability of the ITS2 region was detected in the studied Nitraria species as single-nucleotide substitutions (mainly transitions) and single-nucleotide deletion. Five ribotypes of Nitraria were identified in Russia, Kazakhstan and Tajikistan. These ribotypes formed two haplogroups that belong to the species N. schoberi and N. sibirica, respectively. The high importance of the discriminatory role of the ITS2 spacer in identifying species of the genus Nitraria is confirmed.

Highlights

The genus Nitrаria L. (Nitrariaceae) species are a unique object to study the ways and mechanisms of divergence due to polymorphism and ancient origin
Attempts to resolve the taxonomy of Siberian species of the genus Nitrаria have been made based on karyological [6], morphological [7] characters, but molecular markers have several advantages over them, demonstrating significant differences at the genetic level without environmental factor participation
The work objective is a comparative analysis of the nucleotide variability of the ITS2 region and identification of its significance in the taxonomy of the genus Nitrаria species

Summary

Introduction

The genus Nitrаria L. (Nitrariaceae) species are a unique object to study the ways and mechanisms of divergence due to polymorphism and ancient origin. Attempts to resolve the taxonomy of Siberian species of the genus Nitrаria have been made based on karyological [6], morphological [7] characters, but molecular markers have several advantages over them, demonstrating significant differences at the genetic level without environmental factor participation. The work objective is a comparative analysis of the nucleotide variability of the ITS2 region and identification of its significance in the taxonomy of the genus Nitrаria species.

Results

Conclusion