Using the HPTLC-bioluminescence bacteria assay for the determination of acute toxicities in marine sediments and its eligibility as a monitoring assessment tool

Abstract

For an integrated ecological risk assessment of marine sediment contamination, the determination of target-compound concentrations by e.g. mass spectrometric methods is not sufficient to explain sediment toxicity. Due to the presence of a multitude of environmental contaminants in this complex matrix causing a mixed toxicity, the identification and assessment of main toxicants is a challenge. One approach in identifying main toxicants is the application of effect-directed analysis (EDA). In this study, an EDA approach was developed using high performance thin layer chromatography (HPTLC) coupled to bioluminescence bacteria detection with Aliivibrio fischeri for the determination of marine sediments acute toxicity. In a first attempt, the HPTLC separation was optimized with a fast, two-step gradient to separate main hydrophobic organic contaminant (HOC) classes found in marine sediments. An easy-to-use evaluation script for the resulting bioluminescence inhibition images was programed using R. The developed method was applied to sediment extracts of two different sample sets: (i) Fourteen marine sediment samples from the German Bight representing a wide range of contaminant loads and sediment properties and (ii) sediment samples from a core representing temporal trends of contamination. Results from the HPTLC-bioluminescence bacteria assay were compared to HOC concentrations determined by GC-MS/MS. A correlation of the determined inhibition Γ-values for the PAH inhibition zone to PAH concentrations showed a very good agreement (R2 = 0.91). The results of this study were used to evaluate the suitability of the EDA approach to be used as an assessment tool for marine sediments.