Using the critical body residue approach to determine the acute toxicity of cadmium at varying levels of water hardness and dissolved organic carbon concentrations

Abstract

The linkage between acute adverse effects of cadmium and internal cadmium levels were investigated for the oligochaete worm Lumbriculus variegatus in water at varying degrees of hardness and two different dissolved organic carbon (DOC) concentrations. The LC 50s for the effect of cadmium on the survival of the worms greatly differed depending on water hardness and DOC. We found less variability in internal metal toxicity metrics (lethal residue; LR 50s) than in external toxicity metrics (lethal concentration; LC 50s): LC 50s varied from 2.4 to 66.1 μmol/L, while LR 50s varied only from 226 to 413 μmol/kg wet weight. The cadmium body burden appeared to be independent of exposure conditions. From our experimental data, a critical cadmium body residue (324±78 μmol/kg wet weight) associated with 50% lethality was derived. The protective role of DOC and water hardness against cadmium toxicity was evident.