Using Tetracycline to Evaluate Age Estimation in a Long‐Lived Aquatic Mammal

Abstract

ABSTRACTAge estimation is useful for understanding population parameters and, in many vertebrates, relies on the principle that growth layer groups (GLGs) are deposited annually in specific tissues. In Florida manatees (Trichechus manatus latirostris), GLGs in earbones are used to estimate age at death. Opportunities to validate the rate of GLG deposition in earbones from manatees >15 years old are rare, yet important for ensuring accurate age estimation across the species’ lifespan. Tetracycline injection is a useful method for validating GLG interpretations, particularly when the exact age of an animal is unknown. Since 1997, we collected earbones from 10 manatees that were ≥13–69 years old at death and had been injected with tetracycline 9–37 years before death. The number of years since injection (YSI) was estimated by photographing earbone cross‐sections under ultraviolet light, measuring the distance between the fluorescent tetracycline mark and earbone edge, processing the earbones to visualize GLGs, superimposing the distance to evaluate the marks’ locations relative to GLGs, and counting the GLGs between the mark and earbone edge. Seven earbones had tetracycline marks, although 2 of the marks were dull or discontinuous. On average, estimated YSI was 5.6 (SD = 7.5) years less than the known YSI; however, the error was nearly always ≤2 years for manatees that had been injected <20 years before death, consistent with an annual rate of GLG deposition at younger ages. Resorption (i.e., bone turnover) that obliterated GLGs was likely why YSI was underestimated in old manatees with longer post‐injection intervals, although we cannot exclude the possibility that GLG deposition rate may slow in old age. We discuss how age, extrinsic stressors, life history events, and laboratory processing may affect tetracycline visibility, earbone growth, and GLG interpretation. Our study reinforces the challenges with accurately estimating the age of old individuals in long‐lived mammalian species. © 2021 The Wildlife Society.