Using stable isotopes of plasma, red blood cells, feces, and feathers to assess mature-forest bird diet during the post-fledging period

Abstract

Post-fledging use of early successional habitat by mature-forest birds is well-documented, but the important trophic factors driving this association remain poorly understood. We used stable isotope analysis of plasma, red blood cells, feces, and feathers to determine foraging preferences of three mature-forest bird species captured in 8-year-old clearcuts during the post-fledging period. We did not identify a significant source contribution in any consumer tissue combination using MixSIAR posterior distributions, but the position of consumer tissues in bivariate isotope mixing space suggested that all three mature-forest bird species acted as generalist insectivores during the post-fledging period. Furthermore, estimates of the proportional contribution of fruit to the overall diet were <0.13 for all Scarlet Tanager (Piranga olivacea (J.F. Gmelin, 1789)) tissue types, despite observational evidence of frugivory. We observed significant differentiation in core-niche-space estimates between the two obligate insectivores (Worm-eating Warbler (Helmitheros vermivorum (J.F. Gmelin, 1789)) and Ovenbird (Seiurus aurocapilla (Linnaeus, 1766))), suggesting that clearcuts provided adequate habitat to support the different foraging strategies and invertebrate preferences of both species. By using tissues with short turnover rates to address post-fledging trophic associations of mature-forest birds, we provide a comparison of isotopic values of multiple consumer tissue types in this context.