Using Recombinant Human Granulocyte Colony-Stimulating Factor Plus Dexamethasone Sodium Phosphate Mobilize and Apheresis Granulocyte

Abstract

Objective To explore the effect of mobilization agents and blood cell separator for apheresis granulocyte.Methods From December 2011 to September 2012,52 donors who donated blood in our station were included into this study.Recombinant human granulocyte colony-stimulating factor (G-CSF) plus dexamethasone sodium phosphate were used mobilizing donors' granulocyte,and apheresis granulocyte were performed on the blood cell separator.The mobilization effective and the factors that effected product content were analyzed.Results After using mobilizing agent,the peripheral blood leukocyte and neutrophil counts were significantly increased 4-fold and 7-fold (P＜0.05).In the whole apheresis granulocytes procedure,the average time of (201 ± 83) min,anticoagulated whole blood of (4209±806) mL,anticoagulant of (394±83) mL were used.The average volume of granulocytes products was (283±46) mL,and granulocyte content was (2.18±0.81) × 1010.The average product content of male blood donors [(2.48 ± 0.67) × 1010] was higher than that of female donors [(1.77 ± 0.82) × 1010] (t=3.331,P=0.002).Using univariate analysis,we found that there was a positive correlation between donors' hematocrit (HCT),hemoglobin(Hb),white blood cell(WBC),neutrophilic granulocyte (NEUT) before donation and the amount of granulocytes collected (P=0.000,0.000,0.008,0.001,respectively).Using multivariate analysis,we found regression relations between donors' Hb (P =0.030) and NEUT (P=0.0037) before donation and granulocyte yield.Conclusions Donor's peripheral blood granulocytes could be effective mobilized by using recombinant human G-CSF plus dexamethasone sodium phosphate,and using blood cell separator could collect adequate doses of granulocyte for clinical transfusions. Key words: granulocyte colony stimulating factor,recombinant; granulocyte