Using of Fibrin Sealant on Treatment for Tendon Lesion: Study in Vivo

Abstract

AbstractTendon injuries are among the most common orthopaedic problems with long-term disability as a frequent consequence due to its prolonged healing time. Thus, the study has investigated the effect a new fibrin sealant (FS) derived from the venom of crotalus durissus terrificus in tendon repair. Therefore, 12 animals (±185.8 g) from Wistar lineage, showed average weight ± 185.8 g. All animals received the partial transected tendons and then they were separated in two group a random way: Lesion group (GL) and fibrin sealant group (GS). Immediately after the tendon injuries, 9 µL of fibrin sealant was applicated in each transected tendon, in order to form a stable clot with a dense fibrin network. The methodology of edema analysis was made in three stages: before the partial transected tendons, 24 h after tendon injuries induction and after 21 days of fibrin sealant treatment. The quantification of collagen was by slide colored with picrosirius red. We applied the Kolmogorov–Smirnov test to verify the normality between the groups. For the comparison between the groups the Student’s teste was applied for parametric samples and Mann–Whitney test for non-parametric samples with significance level of p < 0.05. The results showed that there were not significant difference between the groups in the first 24 h before the lesion, but the fibrin sealant treatment for tendon lesion can be promote the reduction of edemas after 21 days. The fibrin sealant group showed similar collagen deposition with the injury group, but with the collagen fibers more organized. The results suggest that the fibrin sealant is effectiveness to treat tendon injuries.KeywordsEdemaCollagenTendon injuryHeterologous fibrin sealantBiopolymer