Using of brown algae Sargassum pallidum as raw materials for extraction of fucoxanthin

Abstract

Fucoxanthin is a widespread carotenoid contained in tissues of brown algae, known by its antitumor activity and ability to suppress cancer cells growth. Its inclusion into diet prevents accumulation of the body fat and regulates level of glucose and insulin in the blood. Fucoxanthin can be considered as a promising nutritional supplement for suppressing obesity. Extraction of fucoxanthin from brown algae Sargassum pallidum is investigated under various conditions. The samples of algae were collected in different sites at Primorye coast in 2018–2019. Two types of the preparations were used ― frozen and dried. The frozen preparations were stored under temperature of –18 оC no more than 30 days. For the dried preparations, fresh algae were dried in a dark room by air fan under temperature of +18–+20 оC and then crushed to powder and stored in dark place under the same temperature no more than 6 months. Content of dry substance in the preparations was 16.4 ± 1.0 % for frozen algae and 93.6 ± 1.0 % for dried algae. Fucoxanthin was extracted from these preparations by method of alcohol extraction using the ethanol water solution in ratio of 1 : 10 during 24 hours in a dark place, then the solution was filtered or centrifuged. Concentration of fucoxanthin in the filtered solution was measured in spectrophotometer relative to a standard solution (ethanol 96 %) with wavelength of 350–800 nm. Yield of fucoxanthin was higher from the frozen preparations, but more ethanol was consumed for its extraction in comparison with extraction from the dried preparations. Besides, the dried preparations were processed during 1 day with the ethanol solution in concentration 96, 88, 84, 80, and 76 %, under various temperature. Yield of fucoxanthin decreased with temperature increasing: the optimal temperature was +7 оC, so the room temperature could not be recommended for extraction. The optimum concentration of ethanol for fucoxanthin extraction was 80 ± 2%.