Using morpho-anatomical characters of leaves and stems for distinguishing Bolboschoenus taxa: a case study of Serbian species

Abstract

The genus Bolboschoenus has been intensively studied from the morphological, ecological and physiological aspects, however, anatomical features of stems, leaves and epidermal structures of leaves have not been investigated sufficiently yet. The main objective of this study was to compare the four Bolboschoenus species growing in Serbia at the anatomical level and to define the most important anatomical characteristics for distinguishing these species. Descriptive, nonmetric multidimensional scaling and canonical discriminant analysis of the anatomical characters of leaves, stems and epidermal structures have been conducted for these purposes. The number and position of sclerenchyma strands in the leaves, the presence of bulliform cells in the epidermis, the number of translucent cell layers under the adaxial epidermis in the midrib area of the lamina, the number of aerial cavities in leaves and stems were the most significant anatomical characters for differentiation of species. The most differentiated species was B. glaucus, while B. laticarpus occupied an intermediate position between B. maritimus and B. planiculmis, on the one hand, and B. glaucus on the other. The combination of the anatomical features of leaves and stems and the epidermal structures had a more excellent taxonomic value than these three individual groups of features individually.