Using immobilized enzymatic membrane reactor to treat synthetic phenol polluted drinking water

Abstract

The catalyzed polymerization of phenol from the synthetic polluted drinking water using immobilized enzymatic membrane reactor (MER) was investigated. Horseradish peroxidase (HRP) immobilized microporous filter was prepared by porous calcium alginate method. The immobilized HRP demonstrates better performance for degradation of phenol. In addition, the experimental parameters including reaction time, pH, HRP dosage, and H2O2 concentration were optimized. In the condition of 0.1 mmol/L initial concentration of phenol,4.78u/ml HRP, nH2O2: nphenol=2:1 and reaction time 10 min at 20 °C and pH 7, the degradation of phenol reached 73.57%. Therefore, the immobilized enzymatic membrane reactor is feasible to treat synthetic phenol polluted drinking water.