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Abstract
Concerns have recently been raised about the treatment and use of hydroxyapatite, an important component of fish scale. The goal of this investigation is to evaluate the feasibility of using hydroxyapatite extracted from fish scale (FHAP) for bone tissue engineering. FHAP or sintered FHAP (sFHAP) was added to chitosan/gelatin (CS/GEL) mixture to prepare membrane. The effect of FHAP and sFHAP on the physical and chemical properties of the membrane and their affinities towards MG63 cells were studied. Accordingly, the prepared CS/GEL/FHAP membrane was characterized by FTIR (ATRFTIR) and SEM. Following sintering at 800°C for 4 h, the CS/GEL/sFHAP membrane had a rougher surface than the CS/ GEL/FHAP membrane. Hydroxyapatite from Sigma (SHAP) was used as a standard and the biological response of MG63 cells on the CS/GEL/sFHAP membrane was determined to exceed that of hydroxyapatite, with higher proliferation and differentiation, as verified by MTT assay and alkaline phosphate assay. Briefly, the biocompatible CS/GEL/FHAP membrane may potentially be useful as a biomaterial for engineering bone tissue.
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