Using High Performance Thin Layer Chromatography-Densitometry to Study the Influence of the Prion [RNQ+] and Its Determinant Prion Protein Rnq1 on Yeast Lipid Profiles.

Justin Hines,Joseph Sherma,Quang Bui

doi:10.3390/separations5010006

Justin Hines, Joseph Sherma

Open Access

https://doi.org/10.3390/separations5010006

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Journal: Separations	Publication Date: Jan 16, 2018
Citations: 7	License type: CC BY 4.0

Affiliation: Lafayette College

Abstract

The baker’s yeast Saccharomyces cerevisiae harbors multiple prions that allow for the creation of heterogeneity within otherwise clonal cell populations. However, in many cases, the consequences of prion infection are entirely unclear. Predictions of prion-induced changes in cell physiology are complicated by pleotropic effects, and detection is often limited to relatively insensitive cell growth assays that may obscure many physiological changes. We previously showed that silica gel high performance thin-layer chromatography-densitometry (HPTLC) can be used to empirically determine prion-induced changes in lipid content in yeast. Here, we conduct pair-wise quantifications of the relative levels of free sterols, free fatty acids, and triacylglycerols [petroleum ether-diethyl ether-glacial acetic acid (80:20:1, v/v/v) mobile phase and phosphomolybdic acid (PMA) detection reagent]; steryl esters, methyl esters, and squalene [hexane-petroleum ether-diethyl ether-glacial acetic acid (50:20:5:1, v/v/v/v) and PMA]; and phosphatidylethanolamine, phosphatidylcholine, and phosphatidylinositol (chloroform-diethyl ether-acetic acid (65:25:4.5, v/v/v) and cupric sulfate-phosphoric acid) in otherwise clonal prion-infected ([RNQ+]) and prion-free ([rnq−]) cells in both stationary- and logarithmic-growth phases. We detected multiple statistically significant differences between prion-infected and prion-free cells that varied by growth phase, confirming our pr evious observations that prions exert distinct influences on cell physiology between stationary- and log-phase growth. We also found significant differences between cells expressing or lacking the Rnq1 protein which forms the [RNQ+] prion, providing new clues to the as yet unresolved normal biological function of this prion-forming protein. This investigation further emphasizes the utility of HPTLC-densitometry to empirically determine the effects of prions and other presumed innocuous gene deletions on lipid content in yeast, and we expect that additional analyses will continue to resolve the physiological effects of prion infection.

Highlights

To properly function, proteins need to be folded in the correct conformation; prions are defined as misfolded forms of proteins that can convert additional normal proteins into this altered form [1].Prions are infectious, being able to self-propagate within a cell and, in the case of microorganisms, from mother cell to daughter cells
We found significant differences between cells expressing or lacking the Rnq1 protein which forms the [RNQ+ ] prion, providing new clues to the as yet unresolved normal biological function of this prion-forming protein. This investigation further emphasizes the utility of high performance thin-layer chromatography-densitometry (HPTLC)-densitometry to empirically determine the effects of prions and other presumed innocuous gene deletions on lipid content in yeast, and we expect that additional analyses will continue to resolve the physiological effects of prion infection
In the Mangold mobile phase, the Rf values of the standards were cholesterol, Lipids were identified by agreement between the Rf values of standard and sample zones in each

Summary

Introduction

Proteins need to be folded in the correct conformation; prions are defined as misfolded forms of proteins that can convert additional normal proteins into this altered form [1].Prions are infectious, being able to self-propagate within a cell and, in the case of microorganisms, from mother cell to daughter cells. Many human neurodegenerative diseases have been linked with either prions or prion-like behavior of proteins, such as Alzheimer’s, Parkinson’s, Huntington’s, and mad cow disease [2,3]. Formation of a prion is not a unique phenomenon in mammals. Proteins with prion-like behaviors are being characterized in other groups of organisms, including filamentous fungi [4], sea slugs [5], plants [6], and most notably baker’s yeast [7]. Starting with the identification of [URE3] and [PSI+ ] (prion forms of proteins Ure and Sup, respectively) [7], nearly a dozen prions have been identified in the baker’s yeast Saccharomyces cerevisiae [8]

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Conclusion