Abstract
.SignificanceSearching analyzable metaphase chromosomes is a critical step for the diagnosis and treatment of leukemia patients, and the searching efficiency is limited by the difficulty that the conventional microscopic systems have in simultaneously achieving high resolution and a large field of view (FOV). However, this challenge can be addressed by Fourier ptychography microscopy (FPM) technology.AimThe purpose of this study is to investigate the feasibility of utilizing FPM to reconstruct high-resolution chromosome images.ApproachAn experimental FPM prototype, which was equipped with or objective lenses to achieve a theoretical equivalent NA of 0.48 and 0.63, respectively, was developed. Under these configurations, we first generated the system modulation transfer function (MTF) curves to assess the resolving power. Next, a group of analyzable metaphase chromosomes were imaged by the FPM system, which were acquired from the peripheral blood samples of the leukemia patients. The chromosome feature qualities were evaluated and compared with the results accomplished by the corresponding conventional microscopes.Results The MTF curve results indicate that the resolving power of the FPM system is equivalent and comparable to the conventional microscope, whereas the performance of the FPM system is close to the conventional microscope. When imaging the chromosomes, the feature qualities of the FPM system are comparable to the results under the conventional lens, whereas the feature qualities of the FPM system are better than the conventional lens and comparable to the conventional lens.ConclusionsThis study initially verified that it is feasible to utilize FPM to develop a high-resolution and wide-field chromosome sample scanner.
Highlights
Leukemia is one category of blood cancers that commonly occur in both female and male patients.[1]
With few high-cost exceptions,[8] this limitation indicates that it is very difficult for the objective lens to simultaneously accomplish a large field of view (FOV) and high spatial resolution, which implies that high-resolution objective lenses will have a much smaller FOV than the low-resolution lens
The contrast value of the Fourier ptychography microscopy (FPM) curve is above 0.7 when the frequency increases to 600 lp∕mm, and the cutoff frequency is about 1066 lp∕mm
Summary
Leukemia is one category of blood cancers that commonly occur in both female and male patients.[1] For the diagnosis and treatment of leukemia, chromosome karyotyping[2] is critically important; it organizes and pairs all chromosomes in order of decreasing length. In this process, cytogeneticists have to search the whole specimen slide and identify the analyzable metaphase chromosomes, which is a time-intensive and tedious operation. Considering that the chromosome images must be obtained with high spatial resolution to ensure enough details for clinical karyotyping, the corresponding high-magnification scanning will be significantly slower as each acquisition only covers a much smaller area of the specimen
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