Abstract
Due to their bilayer structure, liposomes are prevalent model systems for studies of biophysical processes on plasma membranes, which naturally show lipid asymmetry. Phosphatidylethanolamine (PE), for example, occurs mainly in the inner leaflet and phosphatidylglycerol (PG) in the outer leaflet of prokaryotic membranes. Cyclodextrins (cyd) are widely used to extract, insert, or exchange lipids from or into the outer leaflet of liposomes or cell membranes. This strategy is, for example, playing a key role in several protocols to produce lipid-asymmetric liposomes.
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