Abstract
The purpose of the present work was to study effectiveness for early pregnancy diagnosis in cattle of the new enzyme immunoassay (EIA) sandwich kit commercially available based on the measurement of pregnancy-associated glycoproteins (PAGs). 120 Holstein-Friesian cattle of mixed age and parity were comprised from different dairy herds. The pregnant females (n = 68) were diagnosed by ultrasonography at day 35-40 after artificial insemination and confirmed by transrectal exploration at 2-3 months after AI. The non-pregnant females (n = 52) were housed in the absence of males during the experimental period. Blood samples were collected from coccygeal vessels of females into EDTA tubes. The serum was obtained by centrifugation and the serum was stored at - 20 C until assay. The PAG concentrations in pregnant and non-pregnant females were determined in serum by EIA kit. The reproducibility inter- and intra-assay of the PAG-EIA is satisfactory (2.78 and 13.19 %, respectively). The accuracy ( 94.8 %) and the test of parallelism were largely acceptable. No cross-reaction was observed with the different hormones tested at different dilutions. PAG-EIA system gave 100 % sensitivity and negative predictive values. Whereas, specificity and positive predictive value were 91.93 and 71.15 %, respectively. The accuracy of pregnancy diagnosis by PAG-EIA was 87.5 %. In conclusion, the present study shows clearly that the EIA kit can be used to measure PAG in serum cows for the detection of gestation in Algeria. Therefore, this alternative technique could be recommended to replace the radioactive methods in immunoassays to improve the reproductive performances and an efficient tool for reproductive management of dairy cattle.
Highlights
Reproductive management in bovine is an important economic component in the success of a dairy farm
The purpose of the present work was to study effectiveness for early pregnancy diagnosis in cattle of the new enzyme-immunoassay (EIA) sandwich kit commercially available based on the measurement of pregnancy-associated glycoproteins (PAGs)
The values of inter-assay CV are 6.08 and 13.19 %, whereas intra-assay CV is low calculated for PAG-enzyme immunoassay (EIA) (2.78 %)
Summary
Reproductive management in bovine is an important economic component in the success of a dairy farm. Zootechnical performance needs to be assessed in order to achieve good level and positive financial results to become profitable in bovine herd. The reproductive efficiency of dairy cows increases significantly incomes from milk sales and shorter calving intervals in the modern dairy industry worldwide. Detection of pregnancy is an essential key to achieve the optimal calving-to-conception interval in dairy cattle. Pregnancy diagnosis facilitates the detection of non-pregnant cows as early as possible after insemination or early embryonic mortality, and allows them to be re-inseminated. Several techniques are being used to diagnose pregnancy in dairy herd such as transrectal palpation or ultrasonographic examination [1,2,3]
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