Abstract
BackgroundOxidative stress and inflammation play important roles in high-fat diet (HFD) induced kidney damage. Previous studies show that the collagen extracted from the skin of monkfish (Lophius litulon) with pepsin (pepsin-solubilized collagen, PSC) exhibits good biological activities. This study investigates the protective effect of PSCP against chronic kidney injury in HFD-fed mice.MethodsPepsin-solubilized collagen was further hydrolyzed into collagen peptides, and the compound with the best 2,2-diphenyl-1-picrylhydrazyl (DPPH) clearance rate was named pepsin-solubilized collagen peptide (PSCP). A group of mice were fed an HFD for 4 weeks, and then for another 6 weeks PSCP was added to their diet at the amount of either 100 or 200 mg/kg.ResultsPepsin-solubilized collagen peptide treatment (200 mg/kg) reduced the mice's serum levels of uric acid (UA), creatinine (CRE), and blood urea nitrogen (BUN) by 27, 20, and 37%, respectively. This treatment also remarkably improved renal histopathology. Moreover, the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) were increased by 96, 52, and 74%, respectively, and decreased the malondialdehyde (MDA) level by 36%. Additionally, PSCP activated the Nrf2 pathway and inhibited NLRP3 signaling to significantly reduce the levels of inflammatory cytokines IL-1β, IL-6, and TNF-α.ConclusionsOur results indicate that compound PSCP has the potential to prevent or control chronic kidney damage.
Highlights
With an improvement in living standards, unhealthy lifestyle choices such as a high-fat diet (HFD) and the lack of exercise have significantly increased the incidence of obesity-related metabolic diseases [1]
Our results indicate that compound pepsin-solubilized collagen peptide (PSCP) has the potential to prevent or control chronic kidney damage
A previous study has shown that a stress signal network composed of ectopic lipid deposition, lipid metabolism disorders, and oxidative stress in the kidney that is linked to obesity conjointly leads to the occurrence and development of chronic kidney disease (CKD) [6]
Summary
With an improvement in living standards, unhealthy lifestyle choices such as a high-fat diet (HFD) and the lack of exercise have significantly increased the incidence of obesity-related metabolic diseases [1]. A previous study has shown that a stress signal network composed of ectopic lipid deposition, lipid metabolism disorders, and oxidative stress in the kidney that is linked to obesity conjointly leads to the occurrence and development of chronic kidney disease (CKD) [6]. Lipid metabolism disorders, which lead to lipid deposition in the kidney, are one of the common features of many primary and secondary renal diseases [7, 8]. In advanced cases, it progresses to CKD. Lipid metabolism disorders causing CKD have become one of the prime focuses of research studies worldwide. This study investigates the protective effect of PSCP against chronic kidney injury in HFD-fed mice
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