Using Antibody rapid test, RT-qPCR, and RFLP/PCR for detecting Toxoplasma gondii in the placenta of abortion women and determining the genotype in Maysan province, Iraq .

Abstract

Toxoplasma gondii is one of the obligatory intracellular protozoan parasites that are important and widespread in all developing or developed countries. It infects all nucleated cells of warm-blooded animals. This study used the lateral flow immunoassay (LFIA), real-time quantitative polymerases chain reactions (RT-qPCR) for detecting the T. gondii in blood and placental tissues, in addition to the restriction fragment length polymorphism (RFLP) technique to determine the genotypes/strains of T. gondii in Maysan province. The study included 40 participating women, distributed as 22 aborted women and 18 without abortion. The LFIA detected that the seropositive rate of anti-T. gondii IgG is 38.9 % and with the using RT-qPCR technique, the infection rate is 10 % and 0.0 % among placenta and sera. it also, showed that 85.7 % of all T. gondii infections are found in aborted women, compared with 14.3 % without abortion. In contrast, it showed that 63.6 % of T. gondii seronegative are women without abortion, compared to 36.4 % among aborted women. The PCR-RFLP technique has identified the main three genotypes “I, II, and III” of T. gondii in Ammara city, and showed that genotype "I" is the most frequent at 50% (6/12) than genotypes II, 4/12 (33.33 %), and III, 2/12 (16.67 %).