Abstract
Abstract This chapter presents a new approach to functional divergence analysis with the combination of ancestral sequence inference, using the family of animal G-protein subunits as an example. Using the method, the evolutionary trends of two types of functional divergence of amino acid residues after gene duplication are traced. These pieces of evolutionary information are useful for making testable hypotheses about functional divergence between protein subfamilies, such as subtypes of G-protein subunits, which can be verified by further experimentation.
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