Abstract

This study proposed a new mixing method for anaerobic digestion treating animal wastewater using air as gas source of agitation (named “air mixing”) and demonstrated its feasibility by comparing with other mixing modes. The results indicated that the methane production for air mixing was increased by 6.4%, 11.9% and 19.6% compared with biogas mixing, mechanical mixing and no mixing. Air mixing improved the mass transfer and the homogeneous mixing time was shortened from 10 min of mechanical mixing to 1.5 min at the same power input. A transient microaerobic environment was created by air mixing, which increased the hydrolysis efficiency by 1.7–11.4% compared with biogas mixing and facilitated VFAs generation and consumption, as well as promoted the syntrophic relationship between facultative bacteria and hydrogenotrophic methanogens. The relative contribution of the improvement of mass transfer and the reaction of microaerobic environment to methane production was 62.9% and 37.1%, respectively.

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