Using adaptive psychophysics to identify the neural network reset time in subsecond interval timing

Abstract

State-dependent network models of sub-second interval timing propose that duration is encoded in states of neuronal populations that need to reset prior to a novel timing operation to maintain optimal timing performance. Previous research has shown that the approximate boundary of this reset interval can be inferred by varying the inter-stimulus interval between two to-be-timed intervals. However, the estimated boundary of this reset interval is broad (250–500 ms) and remains under-specified with implications for the characteristics of state-dependent network dynamics sub-serving interval timing. Here, we probed the interval specificity of this reset boundary by manipulating the inter-stimulus interval between standard and comparison intervals in two sub-second auditory duration discrimination tasks (100 and 200 ms) and a control (pitch) discrimination task using adaptive psychophysics. We found that discrimination thresholds improved with the introduction of a 333 ms inter-stimulus interval relative to a 250 ms inter-stimulus interval in both duration discrimination tasks, but not in the control task. This effect corroborates previous findings of a breakpoint in the discrimination performance for sub-second stimulus interval pairs as a function of an incremental inter-stimulus delay but more precisely localizes the minimal inter-stimulus delay range. These results suggest that state-dependent networks sub-serving sub-second timing require approximately 250–333 ms for the network to reset to maintain optimal interval timing.