Usefulness of Human Herpes Simplex Virus Type 1 Genotyping for Tracing the Geographical Origins of Unidentified Cadavers

Abstract

Aim: We examined the possibility of tracing the origins of cadavers based on the geographic distribution of genotypes of the latent human herpes simplex virus type 1 (HSV-1). Materials & methods: PCR of the V1 region (667 bp) was performed using DNA from human trigeminal ganglia of 107 Japanese cadavers. The phylogenetic analysis was performed using 16 cadavers in Chiba district and 84 reference sequences in the world. Results: The HSV-1 DNA detection rate was 54.2% and it increased with age. This phylogenetic tree showed four large clusters, types I–IV (African, European, Asian and the mixed-type), and two subclusters (Japanese–Korean type and Japanese–Chinese type) in type III. Conclusion: HSV-1 genotype is possible to trace the geographical origin of unidentified cadavers.