Usefulness of DIGE for the detection of protein profile in retained and released bovine placental tissues

Abstract

IntroductionRegardless intensive research, the etiology and mechanisms of retention of fetal membranes in cows, still require elucidation. In our research approach, difference in gel electrophoresis (DIGE) and matrix-assisted laser desorption/ionization (MALDI) identification were used to obtain first results on protein profile of bovine placental membranes which were properly released or retained for more than 12 h after parturition. MethodsPlacentomes from 6 cows that released placenta and from 6 cows that retained fetal membranes were homogenized, fluorescence labeled and subjected to DIGE. ResultsSelected spots that significantly differed between retained and released placenta as well as spots with constant appearance were identified by MALDI. This allowed identification of the following proteins with high statistical reliability: Transforming growth factor beta 2 – high expression in maternal and fetal part of retained fetal membranes, Short transient receptor potential channel 5 –high expression in maternal part of retained and not retained fetal membranes, Rab GDP dissociation inhibitor beta – high expression in fetal part of retained and not retained fetal membranes, Proline dehydrogenase 2 – similar expression in all examined samples, Ras-related protein Rab-7b –high expression only in maternal part of not retained fetal membranes. DiscussionUp to now, these proteins have not been considered as possibly important molecules for the separation/retention of fetal membranes, but their biological roles may suggest it. Further studies are necessary to establish a full profile of bovine placental proteins and define target molecules that may be involved in separation/retention of fetal membranes.