Abstract
We describe methods to introduce 13C specifically to the C2 or C8 positions of 15N-multilabeled purines, thereby permitting “13C tagging” of all base nitrogens except the amino group of adenosine. These procedures permit the incorporation of two or more 15N-multilabeled monomers into a given DNA or RNA fragment, with and without appropriate 13C tags. This approach increases the number of 15N-nmr resonances that can be unambiguously distinguished and thus the amount of information from a single synthesis and a single nmr experiment. © 1998 John Wiley & Sons, Inc. Biopoly 48: 57–63, 1998
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