Use of titanium dioxide doped multi-wall carbon nanotubes as promoter for the growth, biochemical indices of Sesamum indicum L. under heat stress conditions

Abstract

The behavior of multi-walled carbon nanotubes (MWCNTs) and titanium dioxide nanoparticles (TiO2 NPs) as plant growth enhancers was still unclear; however, in this study, the effects of MWCNTs, TiO2NPs, 5%TiO2@MWCNTs, 10%TiO2@MWCNTs and 15%TiO2@MWCNTs on physical and biochemical contents in Sesamum indicum L. under heat stress conditions were studied. The content of malondialdehyde (MDA) and hydrogen peroxide (H2O2) concentrations were reduced by the spraying MWCNTs and TiO2 NPs on plants. The hydrogen peroxide (H2O2) content was reduced by 49.02% in plants treated with 15%TiO2@MWCNTs while 42.14% reduction was found in plants treated with 10%TiO2@MWCNTs. The proportion of oil and the peroxidase enzyme activity in plants treated with 15%TiO2@MWCNTs were increased by 48.99%, for the oil content, and 2.39 times for POD activity respected to the stressed plants. The proportion of unsaturated fatty acids increased in plants treated with 15%TiO2@MWCNTs, 10%TiO2@MWCNTs and TiO2 NPs by 2.7, 2.52, and 2.09 times, respectively, greater than the control of the Shandweel-3 variety. Finally, plants treated with 15%TiO2@MWCNTs showed increases in seed yield and weight 1000-seeds by 4.42 and 1.67 times, respectively. These findings suggest that TiO2@MWCNTs more effective than separated MWCNTs and TiO2 NPs in improve plant growth. In addition, the cultivar Shandweel-3 showed an improvement in growth indicators more than the Giza-32 cultivar.