Abstract
A procedure is described for the simple scale-up of thin-layer chromatographic (TLC) systems to high-performance liquid chromatography (HPLC), which was developed in order to improve the separations involved in the work-up of synthetic reaction mixtures. The semi-quantitative compositional evaluation of the reaction products was performed by TLC, employing specially developed solvent systems. The retention of solutes was controlled by using TLC as a pilot technique, based on the correlation between TLC and HPLC mobilities. HPLC achieved complete removal of the reaction medium and reagents in addition to isolating the required products. According to the scheme described, single-step isolation and purification of synthetic products was accomplished without the need for any pre-treatment of the sample.
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