Use of the Secreted Proteome of Trametes versicolor for Controlling the Cereal Pathogen Fusarium langsethiae.

Alessia Parroni,Marzia Beccaccioli,Alessandro Infantino,Massimo Reverberi,Marzia Scarpari,Agnese Bellabarba

doi:10.3390/ijms20174167

Alessia Parroni, Marzia Beccaccioli + Show 4 more

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https://doi.org/10.3390/ijms20174167

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Abstract

Fusarium langsethiae is amongst the most recently discovered pathogens of small grains cereals. F. langsethiae is the main producer, in Europe, of T2 and HT-toxins in small grain cereals, albeit often asymptomatic; this makes its control challenging. The European Union (EU) is pushing hard on the use of biocontrol agents to minimize the use of fungicides and pesticides, which are detrimental to the environment and responsible for serious pollution of the soil and superficial water. In line with EU directives (e.g., 128/2009), here we report the use of protein fractions, purified from the culture filtrate of the basidiomycete Trametes versicolor, for controlling F. langsethiae. T. versicolor, a so-called medicinal mushroom which is applied as a co-adjuvant in oncology and other pathologies as a producer of biological response modifiers. In this study, the exo-proteome of T. versicolor proved highly efficient in inhibiting the growth of F. langsethiae and the biosynthesis of the T2 toxin. Results are promising for its future use as a sustainable product to control F. langsethiae infection in cereals under field conditions.

Highlights

Fungi are organisms comprising yeasts, molds and mushrooms and they have been used for long time both in medicine as co-therapy and as food for their nutritional value
In line with European Union (EU) directives (e.g., 128/2009), here we report the use of protein fractions, purified from the culture filtrate of the basidiomycete Trametes versicolor, for controlling F. langsethiae
The preliminary assays concerned the effect of cultural filtrate (CF) of T. versicolor added to PDA mediuTmheipnrPeelitmriidniasrhyeassastaycosnccoenncterrantieodntshoefe0ff.0e4ct%ofwc/uvlatunrdal0.f0il8tr%atwe /(vCoFn) othf eT.gvreorwsictholoorf athdedepdatthooPgDenA Fm. leadnigusmethiinaePientcrui dbiastheedsaatt 2c5on◦cCenfotrrat3i,o5nsanofd07.0d4%ayws a/vftearndin0c.u0b8a%tiwon/v(odnait)h(eFgigruorwethS1o)f. tThheepgatrhoowgtehn oFf.tlhanegpsaetthhioaegeinncruebsautletdedatin25th°eCinfohrib3i,t5ioannodf753d.a8y%s aafttethreinccounbcaetniotrnat(idoani)o(fF0ig.0u4r%e Sw1)/v

Summary

Introduction

Fungi are organisms comprising yeasts, molds and mushrooms and they have been used for long time both in medicine as co-therapy and as food for their nutritional value. Polysaccharides, proteins, glycoproteins and peptides from different mushrooms (i.e., Lentinula edodes, Ganoderma lucidum, Trametes versicolor) demonstrated antibacterial, antiviral, antitumor and immune modulatory activity. Increased melanin content enhancing nrf -2 expression and protecting melanocytes against the dangerous ROS effect (due to the high intrinsic oxidative stress expressed by cancer cells) and, a significant reduction of cell growth [6]. Another group of molecules with medicinal properties, such as proteins and peptides from higher basidiomycetes, has attracted the interest of the scientific community. They are small protein molecules with a molecular weight of about 13kD with immune regulating activity [7] and the number of proteins belonging to FIP family is continuously increasing

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