Use of the restriction enzyme AvaI and exo- Bst polymerase in strand displacement amplification.

Abstract

BioTechniquesVol. 24, No. 3 BenchmarksOpen AccessUse of the Restriction Enzyme AvaI and Exo− Bst Polymerase in Strand Displacement AmplificationMaria A. Milla, Patricia A. Spears, Robert E. Pearson & G. Terrance WalkerMaria A. Milla*Address correspondence to G. Terrance Walker or Maria A. Milla, Department of Molecular Biology, Becton Dickinson Research Center, PO Box 12016, 21 Davis Drive, Research Triangle Park, NC 27709-2016, USA. Internet: E-mail Address: walker@bdrc.bd.com or E-mail Address: milla@bdrc.bd.comBecton Dickinson Research Center, Research Triangle Park, NC, USASearch for more papers by this author, Patricia A. SpearsBecton Dickinson Research Center, Research Triangle Park, NC, USASearch for more papers by this author, Robert E. PearsonBecton Dickinson Research Center, Research Triangle Park, NC, USASearch for more papers by this author & G. Terrance Walker*Address correspondence to G. Terrance Walker or Maria A. Milla, Department of Molecular Biology, Becton Dickinson Research Center, PO Box 12016, 21 Davis Drive, Research Triangle Park, NC 27709-2016, USA. Internet: E-mail Address: walker@bdrc.bd.com or E-mail Address: milla@bdrc.bd.comBecton Dickinson Research Center, Research Triangle Park, NC, USASearch for more papers by this authorPublished Online:15 Aug 2018https://doi.org/10.2144/98243bm12AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinkedInRedditEmail FiguresReferencesRelatedDetailsCited ByRapid, Visual, and Sequence-Specific Detection of Salmonella in Egg Liquid with vis-NEAA, a CRISPR/Cas12 Empowered New Strategy9 February 2022 | Journal of Agricultural and Food Chemistry, Vol. 70, No. 7Strand displacement DNA synthesis by DNA polymerase gp90 exo― of Pseudomonas aeruginosa phage 1Biochimie, Vol. 170Photochemical Acceleration of DNA Strand Displacement by Using Ultrafast DNA Photo-crosslinking29 August 2017 | ChemBioChem, Vol. 18, No. 20Regulation of yeast DNA polymerase δ-mediated strand displacement synthesis by 5′-flaps26 March 2015 | Nucleic Acids Research, Vol. 43, No. 8Optimization and design of oligonucleotide setup for strand displacement amplificationJournal of Biochemical and Biophysical Methods, Vol. 63, No. 3Strand displacement amplification: a versatile tool for molecular diagnostics9 January 2014 | Expert Review of Molecular Diagnostics, Vol. 4, No. 2DNA Polymerases as Engines for BiotechnologyScott C. Hamilton, Joseph W. Farchaus & Maria C. Davis5 September 2018 | BioTechniques, Vol. 31, No. 2SDA Target AmplificationReal-Time, Sequence-Specific Detection of Nucleic Acids during Strand Displacement AmplificationAnalytical Biochemistry, Vol. 276, No. 2Characterization of an Extremely Thermostable Restriction Enzyme, Psp GI, from a Pyrococcus Strain and Cloning of the Psp GI Restriction-Modification System in Escherichia coliApplied and Environmental Microbiology, Vol. 64, No. 10Non–Polymerase Chain Reaction Mediated Target Amplification Techniques Vol. 24, No. 3 Follow us on social media for the latest updates Metrics Downloaded 383 times History Published online 15 August 2018 Published in print March 1998 Information© 2018 Author(s)PDF download