Use of the RAPD Technique for Identification ofFusarium oxysporumf. sp.dianthifrom Carnation

Abstract

The random amplified polymorphic DNA (RAPD) method was used to distinguish among pathogenic and nonpathogenic isolates of Fusarium oxysporum recovered from carnation. Fifty-eight isolates, which were isolated in Israel from different cultivars of carnation, included 42 pathogenic isolates of race 2, one pathogenic isolate of race 4, and 15 nonpathogenic isolates. These isolates were examined for RAPD patterns with 30 arbitrary primers of 10 bases. The RAPD patterns generated by each of 22 primers enabled us to distinguish clearly between pathogenic and nonpathogenic isolates from carnation. The amplification patterns of race 2 isolates were identical for all the primers examined [...]