Abstract
The microvine is a gibberellic acid (GA)-insensitive mutant that exhibits a dwarf nature and short life cycle and is amenable to agrobacterium-mediated transformation. Because of these features and its continuous flowering nature, it is a valuable model system for rapid genetic studies in grapevine. Adaptation of a versatile system combining the use of microvine together with a plant gene switch (PGSS) inducible expression system for controlled expression of transgenes and an artificial microRNA (amiRNA)-based gene silencing method is described. Transformation of microvine using embryonic callus to regeneration of transformed plants took less than 6 months. The inducible expression system could be turned on to induce the accumulation of transgene about 15-fold within 72 h. Functionality of the amiRNA was tested on target transcript accumulation, and a 40% reduction in target mRNA was achieved. The utility of the system combining microvine, gene switch expression, and amiRNA to study genes involved in fruit developmental processes is discussed.
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