Abstract
Noggin (Noggin1) protein inhibits Smad1-dependent TGF-beta signaling pathway by extracellular binding of BMP proteins. Recently we found two previously unknown representatives of Noggin family - Noggin2 and Noggin4. Here we investigate by using the luciferase reporter constructs the ability of Noggin2 to inhibit BMP-, Activin/Nodal- and Wnt-signaling pathways in the early Xenopus laevis embryos. The effectiveness of this inhibitory activity of Noggin2 is comparing with that of well-known inhibitors of the indicated pathways - the secreted proteins Cerberus and Follistatin. Besides revealing of novel properties of Noggin2, our findings demonstrate that luciferase-reporter assay is as a useful tool for signaling pathways investigation in the model of Xenopus embryos.
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