Use of the blocking procedure in mixed hemadsorption tests for the analysis of surface antigens in liver tissue culture cells

Abstract

For detection of surface species-specific and specific tumor antigens in the various tissue culture cell lines, including interspecies hybrid cell line, the blocking procedure was included in a microhemadsorption test (MHT). Use of the appropriate high-titered blocking antisera unadequate to the indicatory system of MHT permits the detection of different species-specific and tumor antigens on the surface of the liver tissue culture cells with the use of heterologous unabsorbed immune sera.