Abstract
Hyoscyamine and scopolamine as free bases were simultaneously quantitated by GLC, using tetraphenylethylene as an internal standard. A linear relationship was found between the ratios of the integrated peak areas of alkaloid to the internal standard and the actual weight ratios of alkaloid to the internal standard for both hyoscyamine and scopolamine. Pure compounds and extracts from Hyoscyamus niger Linne powder were analyzed. The combined precision of the extraction procedure of plant materials with the GLC procedure was determined.
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