Use of STSs and SSRs as rapid and reliable preselection tools in a marker-assisted selection-backcross scheme

Abstract

We describe a new approach for using suitable STS and SSR markers as a powerful molecular tool for screening segregating populations involved in backcross schemes for marker-assisted selection, as a preselection step. Since it can be applied to very large populations, this preselection strategy allows one to increase substantially the pressure of selection at each backcross generation. The technique is fast and reproducible, and can be made even more efficient and costeffective by simultaneous DNA amplification from different primer pairs. In the example illustrated here, three suitable PCR-based markers were used to complete the selection of 300 individuals out of 2300 in less than one month with two people working on the project.