Abstract
Monilinia fructicola, M. laxa and M. fructigena are the causal agents of brown rot of pome and stone fruits. M. fructicola is not present in Europe and is classed as a quarantine pathogen in EU countries. A 418-bp group-I intron has been located in the small subunit (SSU) rDNA gene of M. fructicola which is absent from M. laxa and M. fructigena. PCR primers specific to the 3′-region of the intron together with the SSU rDNA primer NS5 were able to amplify a 444-bp product from M. fructicola and fruit tissue infected with M. fructicola but not from the other two species. This allows for the rapid and sensitive detection of this pathogen in planta.
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