Use of sonication to determine the size distributions of soil particles and organic matter

Abstract

Applying ultrasound energy to soil-water suspensions (sonication) is an established method of determining the size distributions of soil primary mineral particles and associated organic matter. The size distributions may vary, however, with sonication input energy and soil type. The objective of this study was to determine the effects of sonication input energy on the size distributions of soil mineral particles and organic matter for a range of soil textures and carbon contents typical of agricultural soils in southwestern Ontario. The soils included a Brookston clay loam, a Brookston clay, a Huron silt loam, a Perth silt loam and a Harrow sandy loam. All soils were under no-tillage management. Nine sonication energies ranging from 50 to 1500 J mL–1 were applied to soil-water suspensions (1:4 mass ratio), and the soil particle size distribution results were compared with those obtained using the standard chemical dispersion (pipette) method. The three medium- and coarse-textured soils (Huron, Perth, Harrow) required about 250 J mL–1 for complete dissociation of soil aggregates, while the two fine-textured soils (Brookston) required sonication energies of 600-750 J mL–1. Increasing sonication energy increased the amount of soil organic carbon (SOC) measured in the clay-size fraction and decreased the amounts in the sand and silt fractions. Therefore, accurate determinations of particle size distribution and SOC contents require an initial assessment of the amount of sonication energy required for the complete dispersion of the particle size fractions. For the Brookston clay loam and Brookston clay soils, 40–52% less particulate SOC was found in the sand fraction at 750 J mL–1 sonication energy than that obtained using the standard pipette method, indicating particle size reduction by sonication of particle organic matter. It should be noted that the sand-size SOC typically represents a small fraction. Furthermore, sonication had a minor effect on the SOC content of the clay fraction. It was concluded that sonication is a viable technique for determining the size distribution of soil primary mineral particles, as well as the amount of SOC associated with the silt and clay fractions. Key words: Sonication, ultrasound energy, particle size distribution, organic carbon fractionation, clay soil