Abstract
When immune spleen cells packed into glass capillaries were allowed to migrate in the presence of medium containing dengue virus antigen, there was a specific inhibition of the migration of these cells in vitro. This inhibition appears to be due to the production of MIF as the culture supernatant obtained by incubating sensitized spleen cells and dengue antigen was found to contain this factor. When T and B cells were purified from immune spleen cells and lymph nodes, it was observed that only T cells produced MIF thereby suggesting that spleen cell migration inhibition is a specific T-cell mediated immune phenomenon and that dengue virus elicits cell-mediated immunity. The feasibility of using the direct spleen cell migration inhibition test as an in vitro assay to detect CMI in mice is being discussed.
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