Abstract
Outbreaks of infectious diseases may occur in animal and human populations; this underlines the need for suitable One Health approaches. During outbreak situations, straightforward identification of etiological agents is indispensable for taking countermeasures. A recently published protocol for metagenomic virus detection in clinical specimens (TUViD-VM) was developed for snap-frozen tissues which can be challenging to obtain. Here, we describe the use of RNALater®-treated tissue at ambient temperatures for virome sequencing. This study demonstrates that samples stored in RNALater® buffer yield similar results to those stored snap-frozen.
Highlights
This study demonstrates that samples stored in RNALater R buffer yield similar results to those stored snap-frozen
We evaluated the application of the TUViD-VM protocol on samples preserved in RNALater R RNA Stabilization Reagent (Qiagen, Hilden, Germany)
We demonstrated the feasibility of using RNALater R -stored tissue homogenates as basis for metagenomic sequencing
Summary
Specialty section: This article was submitted to Infectious Diseases, a section of the journal Frontiers in Microbiology. Kurth A (2017) Use of RNALater R Preservation for Virome Sequencing in Outbreak Settings. Outbreaks of infectious diseases may occur in animal and human populations; this underlines the need for suitable One Health approaches. Straightforward identification of etiological agents is indispensable for taking countermeasures. A recently published protocol for metagenomic virus detection in clinical specimens (TUViD-VM) was developed for snap-frozen tissues which can be challenging to obtain. We describe the use of RNALater R -treated tissue at ambient temperatures for virome sequencing. This study demonstrates that samples stored in RNALater R buffer yield similar results to those stored snap-frozen
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