Abstract
A novel nucleic acid amplification method, loop-mediated isothermal amplification, was developed and recently demonstrated detection of Newcastle disease virus (NDV) in tissue samples. But slaughter of poultry for test samples is often faced with resentment by low-income farmers. This study was undertaken to determine the test properties of reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) in detection of NDV in clinical cases using cloacal and oropharyngeal swabs. Samples included 46 tracheal tissues, 94 cloacal and 107 oro-pharyngeal swabs from on-station and 30 spleens, 74 cloacal and 74 oro-pharyngeal swabs from the field. Analysis was done using specific RT-LAMP targeting the fusion (F) protein. While the method detected NDV from swab samples, no RNA of other poultry disease viruses was amplified, indicating analytical specificity of 100%. RT-LAMP took ≤36 minutes in 83% (n=329) of positive reactions with all samples amplified in
Highlights
Newcastle disease (ND) is the principal factor limiting rural poultry production in Asian and African countriesincluding Uganda
All chicks in the control group did not show signs or lesions of ND during the experimental period and none had died by time of termination of study
Whereas positive results of cloacal swabs by both ICA and RTLAMP were similar (97.3%), these were lower than 100.0% positive results observed in reverse transcriptase loop-mediated isothermal amplification (RT-loopmediated isothermal amplification (LAMP))
Summary
Newcastle disease (ND) is the principal factor limiting rural poultry production in Asian and African countriesincluding Uganda. 80% of household poultry and is caused by enveloped. RNA viruses of the Avian Paramyxovirus serotype 1. To prevent spread of Newcastle disease virus (NDV) during outbreaks, faster methods of diagnosis are required (IAEA /FAO, 2006). Virus isolation is the gold standard method for detection of NDV, haemagglutination inhibition (HI) test is considered the standard laboratory test for the diagnosis of ND (OIE, 2010). In Uganda, diagnosis of ND is often based on clinical history, clinical signs, lesions and laboratory tests mainly Haemagglutination (HA) and immunochromatographic assay (ICA) strips. Diagnosis by reverse transcriptase polymerase chain reaction
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
