Use of response surface methodology to optimize production of pectinases by recombinant Penicillium griseoroseum T20

Abstract

Abstract Response Surface Methodology (RSM) has been the most utilized optimization method in recent years. Lately, many studies have successfully applied this technique to enzyme production processes of industrial interest. In this context, the production process of pectin lyase (PL) and polygalacturonase (PG) by the genetically modified strain Penicillium griseoroseum T20 was studied. RSM enabled determination of optimal cultivation conditions for PL and PG production. The highest production of PL was obtained after 87.7 h in medium containing sucrose at an initial concentration of 15.7 g/L, and in this condition, the model estimated a PL activity of 2428 U/mL. The highest PG production was obtained after 83.8 h, and in this condition, the model estimated a PG activity of 9465 U/mL. The production of PL and PG between the Penicillium griseoroseum T20 and Penicillium griseoroseum wild type strains was compared after optimization, and increases of more 400 times were observed. Although the RSM presents some limitations, which were addressed in this work, the results show that it was successfully utilized. This provides evidence for great potential of industrial application of the Penicillium griseoroseum T20 strain for the production of PL and PG.