Abstract
FMDV can be shed with milk of infected animals and infect susceptible animals. The possibility of using real-time qRT-PCR for FMD virus detection in cattle milk was assessed. To prepare samples of FMDV infected milk the following strains were used: А No. 2177/ Amursky/2013, О No. 2123/South Ossetia/2011, Asia-1 No. 1987/Amursky/2005, С No. 564, SAT-1 No. 2033/Botswana, SAT-2/Saudi Arabia, SAT-3/Bech 1/65. For specifcity test swine vesicular disease O-72 strain, Vesicular Exanthema of Swine A-48 strain and vesicular stomatitis virus Indiana strain from FGBI “ARRIAH” collection were used. The obtained data showed that real time qRT-PCR can be successfully used for the virus detection in milk of infected cows as well as in epithelium and cell cultures. Analytical sensitivity of real-time qRT-PCR in milk samples is 1–10 TCD50/ml, the method specifcity is 100%. When identifying basic validation characteristics of the method it was proven that real-time qRT-PCR demonstrates precision and sensitivity and can detect FMDV in its genetic diversity. So, the detected data showed that the real-time qRT-PCR is a precious method of milk and dairy product control.
Highlights
FMD is a highly contagious disease of domestic and wild cloven hoofed animals capable of causing great economic damage nationwide
FMD is not endemic in Russia, there is a constant threat of the virus introduction from the neighbo ring Asian countries, primarily from China and Mongolia
FMD outbreaks caused by the infection introduction from other countries were reported in Russia in 1995, 2000, 2004–2006 and 2010–2018 [3]
Summary
FMD is a highly contagious disease of domestic and wild cloven hoofed animals capable of causing great economic damage nationwide. FMD free countries are under constant risk of the disease introduction from endemic regions, such as Asia, Africa and South America. The disease agent is non-enveloped RNA-containing FMDV, Aphthovirus Genus, Picornaviridae family. There are seven types of the virus: О, А, С, Asia-1, SАТ-1, SАТ-2, SАТ-3. Within each type there are many genetic and antigenic variants of the virus. FMD is not endemic in Russia, there is a constant threat of the virus introduction from the neighbo ring Asian countries, primarily from China and Mongolia. FMD outbreaks caused by the infection introduction from other countries were reported in Russia in 1995, 2000, 2004–2006 and 2010–2018 [3]
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