Abstract
The objective of this study was to investigate the potential use of protein hydrolysate from yellow stripe trevally as a nitrogen source for the growth of different microorganisms. Protein hydrolysates from yellow stripe trevally with different degrees of hydrolysis (5, 15 and 25%) prepared using Alcalase (HA) or Flavourzyme (HF) were determined in comparison with commercial Bacto Peptone. For bacteria, Staphylococcus aureus and Escherichia coli, HF with 25% DH (HF25) yielded the highest cell density and specific growth rate (μmax) and the lowest generation time (td) (p 0.05). The pH of culture broth containing HF25 decreased markedly during the first 8 hours of cultivation of S. aureus and E. coli (p 0.05), compared with that containing Bacto Peptone. Scanning electron microscopic study revealed no differences in size and shape of microorganisms cultured in HF25 and Bacto Peptone (p > 0.05).
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