Use of propofol as an anesthetic and its efficacy on some hematological values of ornamental fish Carassius auratus

Hosna Gholipourkanani,Samaneh Ahadizadeh

doi:10.1186/2193-1801-2-76

Abstract

The aim of this study was to determine the level of anesthesia attained in Carassius auratus using a propofol bath administration and using values of haematological profile of blood and examinations, to assess the effects of the fish exposure to that anaesthetic. Acute toxicity values of propofol for gold fish were found 96 h LC50 6.353 mg/L, 96 h LC1 2.966 mg/L and 96 h LC99 13.609 mg/L. Time to induce anesthesia in propofol experiment was significantly higher than Clove oil (p < 0.05), but there was no significant difference in recovery time between the experiments. No significant decrease was found in Total RBC, WBC, HCT, MCH, MCV and leukogram indices (p > 0.05). MCHC (%) level of propofol experiment (13.93 ± 1.36) showed significant (p < 0.05) decrease than Clove oil anesthesia (94.95 ± 24.50) and control (62.46 ± 21.90). Hb(g/dl) content (5.20 ± 0.73) showed decrease in propofol exposure compared with control (15.41 ± 4.76) and clove oil experiment (25.39 ± 5.73) (p < 0.05).

Highlights

In recent years, different types of anesthetics are used to aid in the capture, handling, artificial reproduction, surgery procedures and transport of fish as an anti-stress in modern aquaculture (Roubach et al 2005).A few number of anesthetics have proved effective in anaesthetisation of fish with its own advantages and drawbacks (Velíšek et al 2006)
Based on tests of acute toxicity to gold fish, the 96-hour lethal concentrations of propofol were determined (96 h LC50 6.353 mg/L, 96 h LC1 2.966 mg/L and 96 h LC99 13.609 mg/L). 96-h LC50 propofol of gold fish placed into the higher concentration (16 ppm) initially exhibited irritation, as evidenced by rapidly darting about the aquaria, the mortality of this concentration was 100% after 30 min
The time required to induce anesthesia using propofol was significantly higher than Clove oil (p < 0.05), but there was no significant difference in recovery time between the experiments

Summary

Introduction

Different types of anesthetics are used to aid in the capture, handling, artificial reproduction, surgery procedures and transport of fish as an anti-stress in modern aquaculture (Roubach et al 2005).A few number of anesthetics have proved effective in anaesthetisation of fish with its own advantages and drawbacks (Velíšek et al 2006). Different types of anesthetics are used to aid in the capture, handling, artificial reproduction, surgery procedures and transport of fish as an anti-stress in modern aquaculture (Roubach et al 2005). Only MS-222 (tricaine methanesulfonate) is registered for use on food fish in the U.S and the United Kingdom. Aquaculture industry needs more compounds to be evaluated experimentally (Coyle et al 2004) and introduce on ornamental and food fish. Some anaesthetics reduce or block the activation of the hypothalamic-pituitary-interrenal (HPI) axis associated with stressors and decrease or prevent the release of the stress hormone cortisol to the bloodstream of fish (Hoskonen and Pirhonen 2006). Anesthetics act on the central nervous system in such a way with placing the fish into an anaesthetic solution that is absorbed through the gills and enters the arterial

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