Abstract
Verticillium dahliae, V. albo-atrum, and V. tricorpus are common pathogens of potato. Currently, polymerase chain reaction (PCR) assays based on sequence differences in their ribosomal RNA genes are available for the specific diagnosis of the first two species. Using the same principles, we developed an analogous assay to detect V. tricorpus. The 18-28S rDNA intragenic region of V. tricorpus was obtained by PCR amplification of genomic DNA from the fungus. Sequence analyses indicated that the 5.8S rDNA sequences were conserved among all three Verticillium species but the internal transcribed spacer regions of V. tricorpus clearly were divergent. These sequence differences were used to synthesize a specific primer set for the diagnosis of V. tricorpus [...]
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
